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topo plasmid  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co topo plasmid
    Topo Plasmid, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/topo+blunt+cloning+kit/5+min+TA+Blunt-Zero+Cloning+Kit/pm40441601-184-28-30
    Average 96 stars, based on 299 article reviews
    topo plasmid - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: The landscape of cryptic antisense transcription in human cancers reveals an oncogenic noncoding RNA in lung cancer
    Article Snippet: The full length of CAPT was identified and amplified from the total RNA of NCI-H1299 cells and NCI-H520 by 5′- and 3′-RACE using the SMARTer RACE cDNA Amplification Kit (Takara Bio, USA, catalog no. 634858) following the manufacturer’s protocol.

    Article Title: Pan-cancer transcriptome analysis reveals widespread regulation through alternative tandem transcription initiation
    Article Snippet: Two days after infection, cells were selected and maintained with puromycin (2 μg/ml; Sigma-Aldrich, p8833).

    Polymerase Chain Reaction:

    Article Title: Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper, Sogatella furcifera.
    Article Snippet: Although CRISPR/Cas9 has been widely used in insect gene editing, the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small, have hard shells, and/or are difficult to collect and maintain outside of their normal environment.. Such is the case with Sogatella furcifera, the white-backed planthopper (WBPH), a significant pest of Oryza sativa (rice) that oviposits inside rice stems.. Egg extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice stem.

    Article Title: Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
    Article Snippet: .. Then, the PCR products were purified with a gel extraction kit (TIANGEN, Beijing, China), introduced into the vector in 5 min TOPO-Blunt Cloning Kit (Vazyme, Nanjing, China), and sequenced by the Sanger method (Tsingke, Wuhan, China). ..

    Article Title: The landscape of cryptic antisense transcription in human cancers reveals an oncogenic noncoding RNA in lung cancer.
    Article Snippet: The full length of CAPT was identified and amplified from the total RNA of NCI-H1299 cells and NCI-H520 by 5′- and 3′-RACE using the SMARTer RACE cDNA Amplification Kit (Takara Bio, USA, catalog no. 634858) following the manufacturer ’s protocol. .. The PCR products were subsequently cloned to the PCE2 vector by the TOPO-Blunt Cloning Kit (Vazyme, C602) and were validated by Sanger sequencing. ..

    Cloning:

    Article Title: Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper, Sogatella furcifera.
    Article Snippet: Although CRISPR/Cas9 has been widely used in insect gene editing, the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small, have hard shells, and/or are difficult to collect and maintain outside of their normal environment.. Such is the case with Sogatella furcifera, the white-backed planthopper (WBPH), a significant pest of Oryza sativa (rice) that oviposits inside rice stems.. Egg extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice stem.

    Article Title: A distinct class of pan-cancer susceptibility genes revealed by an alternative polyadenylation transcriptome-wide association study
    Article Snippet: .. Bands were excised and the extracted using TIANgel mini purification kit (Tiangen, cat#: DP209) were cloned into PCE2 vector by 5 min TOPO-Blunt Cloning Kit (Vazyme, cat#: C602) for Sanger sequencing . ..

    Article Title: Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
    Article Snippet: .. Then, the PCR products were purified with a gel extraction kit (TIANGEN, Beijing, China), introduced into the vector in 5 min TOPO-Blunt Cloning Kit (Vazyme, Nanjing, China), and sequenced by the Sanger method (Tsingke, Wuhan, China). ..

    Article Title: The landscape of cryptic antisense transcription in human cancers reveals an oncogenic noncoding RNA in lung cancer.
    Article Snippet: The full length of CAPT was identified and amplified from the total RNA of NCI-H1299 cells and NCI-H520 by 5′- and 3′-RACE using the SMARTer RACE cDNA Amplification Kit (Takara Bio, USA, catalog no. 634858) following the manufacturer ’s protocol. .. The PCR products were subsequently cloned to the PCE2 vector by the TOPO-Blunt Cloning Kit (Vazyme, C602) and were validated by Sanger sequencing. ..

    Transformation Assay:

    Article Title: Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper, Sogatella furcifera.
    Article Snippet: Although CRISPR/Cas9 has been widely used in insect gene editing, the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small, have hard shells, and/or are difficult to collect and maintain outside of their normal environment.. Such is the case with Sogatella furcifera, the white-backed planthopper (WBPH), a significant pest of Oryza sativa (rice) that oviposits inside rice stems.. Egg extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice stem.

    Gel Extraction:

    Article Title: Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
    Article Snippet: .. Then, the PCR products were puri ed with a gel extraction kit (TIANGEN, Beijing, China), introduced into the vector in 5 min TOPO-Blunt Cloning Kit (Vazyme, Nanjing, China), and Page 5/20 sequenced by the Sanger method (Tsingke, Wuhan, China). ..

    Article Title: Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
    Article Snippet: .. Then, the PCR products were purified with a gel extraction kit (TIANGEN, Beijing, China), introduced into the vector in 5 min TOPO-Blunt Cloning Kit (Vazyme, Nanjing, China), and sequenced by the Sanger method (Tsingke, Wuhan, China). ..

    Plasmid Preparation:

    Article Title: Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
    Article Snippet: .. Then, the PCR products were puri ed with a gel extraction kit (TIANGEN, Beijing, China), introduced into the vector in 5 min TOPO-Blunt Cloning Kit (Vazyme, Nanjing, China), and Page 5/20 sequenced by the Sanger method (Tsingke, Wuhan, China). ..

    Article Title: A distinct class of pan-cancer susceptibility genes revealed by an alternative polyadenylation transcriptome-wide association study
    Article Snippet: .. Bands were excised and the extracted using TIANgel mini purification kit (Tiangen, cat#: DP209) were cloned into PCE2 vector by 5 min TOPO-Blunt Cloning Kit (Vazyme, cat#: C602) for Sanger sequencing . ..

    Article Title: Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
    Article Snippet: .. Then, the PCR products were purified with a gel extraction kit (TIANGEN, Beijing, China), introduced into the vector in 5 min TOPO-Blunt Cloning Kit (Vazyme, Nanjing, China), and sequenced by the Sanger method (Tsingke, Wuhan, China). ..

    Article Title: The landscape of cryptic antisense transcription in human cancers reveals an oncogenic noncoding RNA in lung cancer.
    Article Snippet: The full length of CAPT was identified and amplified from the total RNA of NCI-H1299 cells and NCI-H520 by 5′- and 3′-RACE using the SMARTer RACE cDNA Amplification Kit (Takara Bio, USA, catalog no. 634858) following the manufacturer ’s protocol. .. The PCR products were subsequently cloned to the PCE2 vector by the TOPO-Blunt Cloning Kit (Vazyme, C602) and were validated by Sanger sequencing. ..

    Purification:

    Article Title: A distinct class of pan-cancer susceptibility genes revealed by an alternative polyadenylation transcriptome-wide association study
    Article Snippet: .. Bands were excised and the extracted using TIANgel mini purification kit (Tiangen, cat#: DP209) were cloned into PCE2 vector by 5 min TOPO-Blunt Cloning Kit (Vazyme, cat#: C602) for Sanger sequencing . ..

    Article Title: Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
    Article Snippet: .. Then, the PCR products were purified with a gel extraction kit (TIANGEN, Beijing, China), introduced into the vector in 5 min TOPO-Blunt Cloning Kit (Vazyme, Nanjing, China), and sequenced by the Sanger method (Tsingke, Wuhan, China). ..

    Clone Assay:

    Article Title: A distinct class of pan-cancer susceptibility genes revealed by an alternative polyadenylation transcriptome-wide association study
    Article Snippet: .. Bands were excised and the extracted using TIANgel mini purification kit (Tiangen, cat#: DP209) were cloned into PCE2 vector by 5 min TOPO-Blunt Cloning Kit (Vazyme, cat#: C602) for Sanger sequencing . ..

    Article Title: The landscape of cryptic antisense transcription in human cancers reveals an oncogenic noncoding RNA in lung cancer.
    Article Snippet: The full length of CAPT was identified and amplified from the total RNA of NCI-H1299 cells and NCI-H520 by 5′- and 3′-RACE using the SMARTer RACE cDNA Amplification Kit (Takara Bio, USA, catalog no. 634858) following the manufacturer ’s protocol. .. The PCR products were subsequently cloned to the PCE2 vector by the TOPO-Blunt Cloning Kit (Vazyme, C602) and were validated by Sanger sequencing. ..

    Sequencing:

    Article Title: A distinct class of pan-cancer susceptibility genes revealed by an alternative polyadenylation transcriptome-wide association study
    Article Snippet: .. Bands were excised and the extracted using TIANgel mini purification kit (Tiangen, cat#: DP209) were cloned into PCE2 vector by 5 min TOPO-Blunt Cloning Kit (Vazyme, cat#: C602) for Sanger sequencing . ..

    Article Title: The landscape of cryptic antisense transcription in human cancers reveals an oncogenic noncoding RNA in lung cancer.
    Article Snippet: The full length of CAPT was identified and amplified from the total RNA of NCI-H1299 cells and NCI-H520 by 5′- and 3′-RACE using the SMARTer RACE cDNA Amplification Kit (Takara Bio, USA, catalog no. 634858) following the manufacturer ’s protocol. .. The PCR products were subsequently cloned to the PCE2 vector by the TOPO-Blunt Cloning Kit (Vazyme, C602) and were validated by Sanger sequencing. ..



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